ABSTRACT
Objective:To investigate the effects of subanesthetic concentration of sevoflurane on the plasticity of dendritic spines in the prefrontal cortex neurons of juvenile rats.Methods:Thirty-six clean-grade male Sprague-Dawley rats, aged 24 days, weighing 50-60 g, were divided into control group (group C) and sevoflurane anesthesia group (group S) using a random number table method, with 18 rats in each group.Group S inhaled 1.2% sevoflurane and 50% oxygen (flow rate 1 L/min) for 3 h, while group C inhaled 50% oxygen (flow rate 1 L/min) for 3 h. Open-field test and Morris water maze test were performed at 3 days after anesthesia.Animals were sacrificed, and brain samples were then taken for determination of the number of apoptotic neurons in layer Ⅱ-Ⅲ of the prefrontal cortex, density of dendritic spines, and expression of postsynaptic density protein 95 and gephyrin by TUNEL staining, Golgi staining or Western blot.Results:Compared with group C, no significant change was found in total distance or time of staying at the central region in the open-field test or the average swimming velocity, escape latency or the number of apoptotic neurons in the Morris water maze test ( P>0.05), and the density of dendritic spines was significantly increased, and the expression of postsynaptic density protein 95 and gephyrin was up-regulated in group S ( P<0.05). Conclusion:Subanesthetic concentration of sevoflurane can enhance the plasticity of dendritic spines in the prefrontal cortex neurons of juvenile rats.
ABSTRACT
Objective To investigate the clinical efficacy of modified cistanche decoction combined with lactulose oral liquid in the treatment of patients with constipation -type irritable bowel syndrome ,and to observe its effect on improving the quality of life of patients.Methods From June 2013 to June 2016,the clinical diagnosis and treatment of 76 patients with constipation-predominant irritable bowel syndrome in the People's Hospital of Qingyuan County were retrospectively analyzed.Among them,lactulose oral liquid was used in the control group.On the basis of the control group,cistanche soup was given in the treatment group ,the control group and the treatment group had 38 cases.The clinical effects,adverse reactions were analyzed ,and before and after treatment ,TCM syndrome score and quality of life score were recorded.Results After treatment,the TCM syndrome scores of the treatment group were significantly lower than those of the control group (t＝2.87,3.17,2.68,3.75,4.27,3.83,5.02,4.40,2.48, P＝0.00,0.00,0.00,0.00,0.00,0.00,0.00,0.00,0.01),the quality of life dimensions scores of the treatment group were significantly higher than those of the control group (t＝3.10,3.44,3.36,3.58,4.51,3.32,2.95,2.89, P＝0.00,0.00,0.00,0.00,0.00,0.00,0.00,0.00).However,after treatment,the scores of TCM syndromes in both two groups were significantly lower than those before treatment ( control group:t ＝4.28,5.11,4.07,6.38,5.06, 2.11,7.32,4.38,20.22,P＝0.00,0.00,0.00,0.00,0.00,0.00,0.04,0.00,0.00;treatment group:t ＝7.81, 9.86,7.68,13.76,9.31,6.57,14.639.62,7.36,P＝0.00,0.00,0.00,0.00).The scores of all dimensions of life quality were significantly higher than those before treatment (control group:t＝2.85,3.59,2.58,2.60,4.01,4.88, 3.05,2.36,P＝0.00,0.00,0.01,0.01,0.00,0.00,0.00,0.02;treatment group:t＝5.57,6.72,6.60,6.40,7.74, 7.63,5.84,6.30,P＝0.00,0.00,0.00,0.00,0.00,0.00,0.00,0.00).The total effective rate of the treatment group was 86.84%,which was significantly higher than 65.79% of the control group(χ2＝7.13,P＝0.02).There were no significant adverse reactions during treatment.Conclusion Cistanche decoction combined with lactulose oral solution for constipation -irritable bowel syndrome patients has significant effect ,and can help to improve the quality of life of patients.